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SRX3225990: GSM2795973: SMAD2/3, BT549, TGFβ 1.5h_ChIP; Homo sapiens; ChIP-Seq
1 ION_TORRENT (Ion Torrent PGM) run: 33.4M spots, 1.7G bases, 888.7Mb downloads

Submitted by: NCBI (GEO)
Study: SMAD2/3 binding pattern in triple-negative breast cancer cell lines, Hs578T and BT549 [ChIP-seq]
show Abstracthide Abstract
SMAD2 and SMAD3 have diverse binding profiles in triple-negative breast cancer cell lines. Overall design: SMAD2/3 ChIP-seq analyses were performed in 2 different TNBCs at 2 different time points after TGFß treatment.
Sample: SMAD2/3, BT549, TGFβ 1.5h_ChIP
SAMN07714512 • SRS2550599 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Ion Torrent PGM
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: SINGLE
Construction protocol: Chromatin isolation, sonication and immunoprecipitation using anti-SMAD2/3 antibody (#610843, BD Transduction Laboratories) were performed essentially as described (Isogaya et al., 2014; Morikawa et al., 2016). The libraries were constructed as described (Murai et al, 2015), using IonXpress Plus Fragment Library Kit (Thermo Fisher Scientific). Adaptor-ligated samples were amplified by 15 cycles of PCR and purified by E-Gel SizeSelect (Thermo Fisher Scientific).
Experiment attributes:
GEO Accession: GSM2795973
Links:
Runs: 1 run, 33.4M spots, 1.7G bases, 888.7Mb
Run# of Spots# of BasesSizePublished
SRR611115233,376,5571.7G888.7Mb2017-11-22

ID:
4535056

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